【英文标准名称】:StandardGuideforConductingStaticAcuteToxicityTestswithEchinoidEmbryos
【原文标准名称】:用海胆纲胚胎做静态剧毒试验的标准指南
【标准号】:ASTME1563-1998(2004)e1
【标准状态】:现行
【国别】:
【发布日期】:1998
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E47.01
【标准类型】:(Guide)
【标准水平】:()
【中文主题词】:实验设计;毒理学;海上环境;海胆纲;剧毒试验;EC50试验;海胆;港湾环境;四十;寿命周期;九十;饼海胆;静态剧毒试验;静态毒性试验;LC50试验;毒性;盐水;曝光试验;水产有机物;用海胆纲胚胎做;用海胆纲胚胎做;八时试验;胚胎开发;普鲁丘斯阶;六时试验;试验
【英文主题词】:acutetoxicitytest;Arbaciapunctulata;Dendrasterexcentricus;echinoid;EC50test;embryodevelopment;marinewaterquality;pluteuslarva;sanddollar;seaurchin;sedimenttest;statictoxicitytest;Strongylocentrotusdroebachiensis
【摘要】:1.1Thisguidecoversproceduresforobtaininglaboratorydataconcerningtheacuteeffectsofatestmaterialonembryosandtheresultinglarvaeofechinoidembryos(seaurchinsandsanddollars)duringstatic48-to96-hexposures.TheseprocedureshavegenerallybeenusedwithU.S.EastCoast(ArbaciapunctulataandStrongylocentrotusdroebachiensis)()andWestCoastspecies(Strongylocentrotuspurpuratus,S.droebachiensis,andDendrasterexcentricus)().ThebasicproceduresdescribedinthisguidefirstoriginatedinJapanandScandanavia(),andparallelprocedureshavebeenusedwithforeignspecies,especiallyinJapanandtheMediterranean().Theseprocedureswillprobablybeusefulforconductingstaticacutetoxicitytestswithembryosofotherechinoidspecies,althoughmodificationsmightbenecessary.1.2Othermodificationsoftheseproceduresmightbejustifiedbyspecialneedsorcircumstances.Althoughusingproceduresappropriatetoaparticularspeciesorspecialneedsandcircumstancesismoreimportantthanfollowingprescribedprocedures,theresultsoftestsconductedbyusingunusualproceduresarenotlikelytobecomparablewiththoseofmanyothertests.Thecomparisonofresultsobtainedbyusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingacutetestsstartingwithembryosofechinoids.1.3Theseproceduresareapplicabletomostchemicals,eitherindividuallyorinformulations,commercialproducts,orknownmixtures.Withappropriatemodifications,theseprocedurescanbeusedtoconductacutetestsontemperature,dissolvedoxygen,andpHandonsuchmaterialsasaqueouseffluents(seealsoGuideE1192),leachates,oils,particulatematter,surfacewatersandsediments().Renewaltestsmightbepreferabletostatictestsformaterialsthathaveahighoxygendemand,arehighlyvolatile,arerapidlytransformedbiologicallyorchemicallyinaqueoussolution,orareremovedfromtestsolutionsinsubstantialquantitiesbythetestchambersororganismsduringthetest.1.4Resultsofacutetoxicitytestswithechinoidembryosshouldusuallybereportedasthe50%effectconcentration(EC50)basedonthetotalabnormallydevelopedembryosandlarvae.Insomesituations,itmightonlybenecessarytodeterminewhetheraspecificconcentrationisacutelytoxictoembryosorwhethertheEC50isaboveorbelowaspecificconcentration.1.5Thisguideisarrangedasfollows:1.6ThevaluesstatedinSIunitsaretoberegardedasthestandard.1.7Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitations.SpecificprecautionarystatementsaregiveninSection7.
【中国标准分类号】:Z16
【国际标准分类号】:
【页数】:22P.;A4
【正文语种】: